![]() The separation of isozymes on the basis of surface charge (and to a lesser extent on molecular weight) may be achieved by electrophoresis in starch gel, acrylamide gel, agarose, cellulose acetate or Cellogel under conditions of pH, ionic strength, and ionic composition appropriate for a specific enzyme. 81 (a) Isozymes resulting from multiple gene loci coding distinctly different polypeptide chains (b) isozymes arising as a result of multiple alleles at a single locus and (c) isozymes which are formed as a result of secondary modifications, which includes covalent modifications, conformational isomers, and the formation of monomers, dimers, trimers, etc., of a basic subunit which may be composed of more than one polypeptide chain. 81 Three major categories of isozymes are tabulated by Harris. Isozymes are defined as multiple molecular forms of an enzyme demonstrating similar or identical catalytic properties. Ruddle, in Tissue Culture, 1973 Isozymes. ![]()
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